Chromosome - encoded f - lactamases of Citrobacter diversus
نویسنده
چکیده
* Universita degli Studi dell'Aquila Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Cattedre di Chimica Biologica e Biologia Molecolare, Localita Collemaggio, 1-67 100 L'Aquila, Italy, t Laboratoire d'Enzymologie et Service de Microbiologie, Universite de Liege Institut de Chimie, B6, B-4000 Sart Tilman (Liege), Belgium, and I Rijksuniversiiteit-Gent Laboratorium voor Microbiologie, Ledeganckstraat 35, B-9000 Gent, Belgium
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Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties.
Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicil...
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